The in-built Fatigue tool in Mechanical does not take into account mean stress from a static analysis. However you can use the ANSYS nCode Designlife add-on. This does allow adding a static analysis result to account for mean stress effects when doing PSD fatigue calculations. However note that ANSYS nCode Designlife does not directly use the PSD results created by ANSYS solver. Instead you provide a harmonic analysis results with unit load and the PSD curves. Additionally you also point to the static results (for mean stress effects).
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