yes 5000. It is an open beaker having PVA and there is a turrax agitator inside attached is the picture of my experimental setup. I varied the speed of the agitator. and added PEI with the help of syninge at 0.333 ml/min.

I want to determine the particle sizes of PEI.

I didn't calculated TSR.

what settings should i use ?

i shall be thankful for your time 

kind regards,